Dna Lab Part 3 And 4

In the nex two days, my lab group and I used alcohol to break through the nucleus of cells and collect DNA, dye the DNA, weigh down the DNA, transfer the DNA into the gel block, And run the experiment. There was a power outage and our wire rusted so we got no results.

We followed these steps: 

1. Open and slightly tilt the tube and pour (5 ml) of the chilled 95 percent ethanol down the side of the tube so that it forms a layer on the top of your soapy solution.
2. Allow tube to stand for 1 minute.
3. Use .2 ml pipette to pull 0.25 ml DNA from the top of the test tube into a micro test tube. Your DNA should stay solid in this solution.
4. Using the disposable pipettes , add 0.25 ml methylene blue solution for each DNA sample
5. Add one drop of glycerin to DNA/methylene blue solution.
6. Make new buffer solution (The buffer should be a 1% solution of baking soda. To make this, combine 2 grams (g) of baking soda with 200 mL of bottled water in one of your bowls and stir well.
7. Pour your buffer solution over the solidified gel. Add enough buffer to submerge the gel.
8. Gently pull the comb out of the gel. Be sure not to remove the comb until you are sure that the agarose gel is completely set. The resulting wells will be used as reservoirs for your samples.
9. Using the butter knife, carefully cut a thin slice of the gel from the top and the bottom to make room for the electrodes.
10. Re-attach the steel wire electrodes.
11. Using a .2 ml pipette, fill each well in the gel with a each suspects DNA solution
12. Using the alligator clip leads, attach the battery pack to the wires resting on the gel chamber. The positive terminal of the battery pack should be connected to to the clip farthest away from the DNA samples.
13. Set the machine to 150 V. DO NOT TOUCH OR DISTURB BOX DURING THIS PROCESS.
14. You should see bubbles forming around the electrodes in the buffer as the current passes through them.
15. Consider using time-lapse to record your results.